Several recent studies have reported a significantly greater prevalence of Clostridium perfringens encoding the novel pore-forming netF toxin gene in dogs with acute hemorrhagic diarrhea syndrome.However, the presence of netF in other canine diarrheal diseases creta girl wig remains poorly characterized.This retrospective, cross-sectional study aimed to describe the prevalence and abundance of netF-positive C.perfringens in fecal samples from 352 dogs with acute and chronic gastrointestinal diseases.Dogs were divided into five groups: acute hemorrhagic diarrhea syndrome (AHDS), acute diarrhea (AD), chronic enteropathy (CE), exocrine pancreatic insufficiency (EPI), and healthy controls (HCs).
The abundances of C.perfringens 16S rRNA, the C.perfringens enterotoxin gene and the C.perfringens netF gene in fecal samples were analyzed by quantitative polymerase chain reaction.In total, 7 of 15 (46.
7%) dogs with AHDS, 10 of 75 (13.3%) dogs with AD, 2 of 120 (1.7%) dogs with CE, 1 of 12 (8.3%) dogs with EPI, and 1 the beast sullivan supply of 130 (0.8%) HC dogs tested positive for netF.
This study provides further evidence that NetF may be a significant contributor to the etiology of AHDS and potentially to a subset of acute nonhemorrhagic diarrhea cases, while it was only rarely detected in chronic gastrointestinal disease phenotypes.